Identification of a Specific Pseudo attP Site for Phage phiC3 Integrase in the Genome of Chinese Hamster in CHO-K1 Cell Line
نویسندگان
چکیده
منابع مشابه
identification of a specific pseudo attp site for phage phic3 integrase in the genome of chinese hamster in cho-k1 cell line
background: phic31 integrase is a dna site-specific recombinase integrates dna into the chromosomes between the two sites of attb and attp. several pseudo attps have been identified in mammalian genomes with critical features for long-term expression of transgene. in this manuscript, we report a novel intrinsic pseudo attp site named chol1 in the chinese hamster genome implementing an inverse p...
متن کاملIdentification of a Specific Pseudo attP Site for Phage PhiC31 Integrase in Bovine Genome
Background: PhiC31 integrase system provides a new platform in various felid of research, mainly in gene therapy and creation of transgenic animals. This system enables integration of exogenous DNA into preferred locations in mammalian genomes, which results in robust, long-term expression of the integrated transgene. Objectives: Identification of a novel pseudo attP site. Materials and Methods...
متن کاملidentification of a specific pseudo attp site for phage phic31 integrase in bovine genome
background: phic31 integrase system provides a new platform in various felid of research, mainly in gene therapy and creation of transgenic animals. this system enables integration of exogenous dna into preferred locations in mammalian genomes, which results in robust, long-term expression of the integrated transgene.objectives: identification of a novel pseudo attp site.materials and methods: ...
متن کاملcreation of tenecteplase-producing cho cell line using site-specific integrase from the phage φc31
background: the aim of this study was to produce a stable cho cell line expressing tenecteplase. materials and methods: in the first step, the tenecteplase coding sequence was cloned in a pdb2 vector containing attb recognition sites for the phage φc31 integrase. then, using lipofection, the cho cells were co-transfected with constructed recombinant plasmid encoding tenecteplase and attb recogn...
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ژورنال
عنوان ژورنال: Iranian Journal of Biotechnology
سال: 2013
ISSN: 1728-3043,2322-2921
DOI: 10.5812/ijb.9301